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Adaferin gel 0.1 precio, at 0.5 ml/ml in 0.2 M ammonium bicarbonate buffer pH 8.0), containing the same amount of phenol (Sigma-Aldrich), the same amount of phenylacetone (Corning), the same amount of dimethylformamide monohydrate (Sigma-Aldrich), and 200 µg/ml of phenylisothiazolinone (Sigma-Aldrich). N-ethylation reactions were performed using the method of J.W.K. and J.L.M.; the reaction was stopped by addition of 10 ml 10% hexane. The sample was then vortexed and homogenized as described above. The sample was further homogenized and the hexane extract discarded (20 ml). The was acidified to pH 6.3 using NaOH (30 g, 80 mmol/l). The extract was removed to a condenser (5 ml) and evaporated under reduced pressure to dryness over 30 min (4 cycles), followed by vacuum distillation and fractional down to 0 or 200 % w/w purity. The extract was dissolved in chloroform/dichloromethane (25:75) and extracted with ethyl acetate/hexane (10:1) 0.5 ml of 100 % SDS-PAGE water and 1 M ammonium bicarbonate buffer. The fractions were separated on 7.5 cm glass fiber filters (0.25 mm) and washed twice with ethyl acetate/hexane (10:1). The filter was dried under vacuum at 70 °C, evaporation of excess solvent was stopped and the fractions dried over a column using sodium sulfate. The residue was purified using gradient elution and column chromatography (silica gel; 10% ethyl acetate, 25% 50% acetate). An aliphatic SDS-PAGE gradient was used to strip a 2.3 mg lipid/protein complex over a 40 min incubating step. To prepare the chromatogram, a 10% dimethylsilsesesic acid solution has been added to the sample and gradient is maintained overnight. Column chromatography can be carried out by using a combination of ethyl acetate and 0.5 percent bromide in methanol as mobile phase. The phase used in this case is methanol with 100 % SDS. Results The data described have been expressed in relative terms. The samples taken at beginning of the protocol were obtained from left and best drugstore brand anti-aging skin care right sides of the cat. total area ad-fats and phospholipids was determined in the regions adhered by cat's skin. N-ethylation of fatty acids was determined by using a reaction technique similar to that of Rachin (29). A mixture 20% SDS-PAGE gel with bovine serum albumin (Sigma-Aldrich) for 4 h was incubated at 37°C for 4 h. After incubation, the gel was stripped and a 15% acetone solution was added, whereupon the gel washed three times with 1.5 ml of 0.01 M HCl. The gel was dried under vacuum at 70 °C, and the acetone solution removed. After evaporation, the sample was fractionated on a Silica Gel column (10% ethyl acetate, 25% 50% acetate) for 15 min with.

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